NADAL Mononucleosis

General information about the test: NADAL C. difficile Toxins A&B Test is a rapid chromatographic immunological test for qualitative determination of the presence of Clostridium difficile Toxins A and Bantigens in human faeces samples. This test aids diagnosis of Clostridium difficile infection and is designed for professional use only. Reagents and materials supplied: 10 NADAL C. difficile Toxins A&B test cassettes 10 specimen collection vials with buffer 1 package insert Test time: 10 min Performance characteristics: NADAL C. difficile Toxins A&B Test shows no cross-reactivity with other microorganisms occasionally present in human faeces ( E.Coli O157:H7, Campylobacter spp , Helicobacter pylori , Salmonella spp , Shigella spp , Listeria monocytogenes , Staphylococcus aureus , Yersinia spp etc ). Sensitivity: >99% Specificity: >99% Test principles: NADAL C. difficile Toxins A&B Test is qualitative immunological test for detection of C. difficile in human faeces samples. NADAL C. difficile Toxins A&B test cassette includes 2 test lines for detection of C. difficile Toxin A or B antigenes respectively. The sample tested binds to the anti-Toxin A and anti-Toxin B antibodies on the conjugate pad and moves further to the test line where in the case of positive result, specific antibodies bound there interact with the mixture conjugates to generate one or two colored lines. It is vital to obtain green coloration in the control (C) region of the test cassette – it serves as an internal test control which proves that sufficient volume of the sample was added and thatproper membrane wicking has occurred. Clinical significance: Clostridium difficile is anaerobic gram-positive spore-forming bacterium – a feature that enables it to persist in patients and physical environments for long periods of time. Infection with C. difficile takes place by fecal-oral route. C. difficile is the main pathogen related to cases of antibiotics associated diarrhea and/or pseudomembranous colitis in hospitalized patients. Colonic microflora of a healthy adult is resistant to C. difficile infection. When the normal colonic flora is compromised resistance to C. difficile is lost . Such conditions might occur after treatment with broad-spectrum antibiotics such as penicillins, cephalosporins and clindamycin. C. difficile releases two high-molecular-weight toxins – A and B. They cause the clinical manifestations of C. difficile infection – ranging from mild self-limited watery diarrhea to fulminant pseudomembranous colitis, toxic megacolon and death.