ELISA DMT Enzyme (DpnI)

Product Details This product is an improved version of DpnI restriction enzyme, exhibiting higher activity compared with conventional DpnI enzyme. This enzyme can effectively recognize and cleave the sequence GmATC (A is methylated) and cannot cleave the sequence GATC (A is not methylated). DMT Enzyme is compatible with reaction buffers of mµLtiple PCR enzymes (such as EasyPfu, TransStart, FastPfu, etc.). After PCR, this enzyme can be added directly to the reaction system to start digestion reaction. After digestion reaction, downstream transformation experiments can be performed without heat inactivation. Restriction Site Highlights High efficiency, high specificity, no star activity. Applications In vitro site-directed DNA mutagenesis; degradation of methylated plasmid template Storage at -20°C for two years Shipping Dry ice (-70 ℃) Product Contents Component GD111-01 GD111-02 DMT Enzyme 200 units 5×200 units 10×FlyCut® Buffer 250 μl 1 mL 6×DNA Loading Buffer 1 mL 1 mL