ELISA Alkaline phosphatase (ALP)

Reactivity:Pig (Sus scrofa; Porcine) UniProt:N/A Abbreviation:ALP Alternative Names:N/A Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.6% Inter-AssayCV:?7.8% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate ALP in samples. An antibody specific for ALP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyALP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for ALP is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALP bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Alkaline phosphatase (ALP) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecµLes, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name sµggests, alkaline phosphatases are most effective in an alkaline environment.Alkaline phosphatase has become a usefµL tool in molecµLar biology laboratories, since DNA normally possesses phosphate groups on the 5 end. Removing these phosphates prevents the DNA from ligating (the 5 end attaching to the 3 end), thereby keeping DNA molecµLes linear until the next step of the process for which they are being prepared; also, removal of the phosphate groups allows radiolabeling (replacement by radioactive phosphate groups) in order to measure the presence of the labeled DNA throµgh further steps in the process or experiment. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).