ELISA Alpha-melanocyte stimulating hormone (A-MSH)

Reactivity: (Homo sapiens) UniProt:N/A Abbreviation:A-MSH Alternative Names:?-MSH; Intermedins; Alpha-Melanotropin, Alpha-Melanocortin; Alpha-Intermedin Application:ELISA Range:123.5-10000 pg/mL Sensitivity:46.5 pg/mL Intra-AssayCV:?3.9% Inter-AssayCV:?6.1% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate A-MSH in samples. An antibody specific for A-MSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyA-MSH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for A-MSH is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of A-MSH bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Melanocyte-stimµLating hormone belongs to a group called the melanocortins. This group includes ACTH, alpha-MSH, beta-MSH and gamma-MSH; these peptides are all cleavage products of a large precursor peptide called pro-opiomelanocortin (POMC). Alpha-MSH is the most important melanocortin for pigmentation.Alpha-MSH coµLd be a potent biological marker for the diagnosis of CFS. Despite extensive research, no reliable biological marker for chronic fatigue syndrome (CFS) has yet been identified. However, hyperactivation of melanotrophs in the pituitary gland and increased levels of plasma alpha-melanocyte-stimµLating hormone (alpha-MSH) have recently been detected in an animal model of chronic stress. Because CFS is considered to be caused partly by chronic stress events, increased alpha-MSH plasma levels may also occur in CFS patients. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).