ELISA Alpha- (1,3)-fucosyltransferase 11 (FUT11)

Reactivity: (Homo sapiens) UniProt:Q495W5 Abbreviation:FUT11 Alternative Names:MGC119338; MGC119339; MGC33202; Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.058 ng/mL Intra-AssayCV:?5.5% Inter-AssayCV:?7.6% Recovery:0.84 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate FUT11 in samples. An antibody specific for FUT11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFUT11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for FUT11 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FUT11 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Fucosyltransferase 11 belongs to the glycosyltransferase 10 family. A fucosyltransferase is an enzyme that transfers an L-fucose sµgar from a GDP-fucose (guanosine diphosphate-fucose) donor substrate to an acceptor substrate. The acceptor substrate can be another sµgar such as the transfer of a fucose to a core GlcNAc (N-acetylglucosamine) sµgar as in the case of N-linked glycosylation, or to a protein, as in the case of O-linked glycosylation produced by O-fucosyltransferase. There are various fucosyltransferases in mammals, the vast majority of which, are located in the Golgi apparatus. The O-fucosyltransferases have recently been shown to localize to the endoplasmic reticµLum (ER).Fucosyltransferase activity may be a risk factor for urinary tract infection. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).