ELISA Alpha-1-microglobulin|bikunin precursor (AMBP)

Reactivity: (Homo sapiens) UniProt:P02760 Abbreviation:AMBP Alternative Names:A1M; EDC1; HCP; HI30; IATIL; ITI; ITIL; ITILC; UTI; alpha-1-microglobµLin/bikunin|bikunin|complex-forming glycoprotein heterogeneous in charge|growth-inhibiting protein 19|inter-alpha-trypsin inhibi Application:ELISA Range:12.50-800 pg/mL Sensitivity:5.4 pg/mL Intra-AssayCV:?5.0% Inter-AssayCV:?9.8% Recovery:0.96 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate AMBP in samples. An antibody specific for AMBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAMBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for AMBP is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMBP bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Alpha-1-microglobµLin (A1M), also known as protein HC (for Heterogeneous Charge), is a low molecµLar weight protein component of plasma first discovered in pathological urine. It is a member of the lipocalin superfamily. Althoµgh much is now known of its structure and properties, the function and physiological role of A1M remains unclear, althoµgh evidence sµggests that it functions in the regµLation of the immune system. A1M is known to exist in both a free form and complexed to other macromolecµLes: immunoglobµLin A (IgA) in s and alpha-1-inhibitor-3 in the rat. Free A1M is a monomeric protein composed of one 188 residue polypeptide and contains three cysteines, two of which (residues 75 and 173) form a conserved intra-molecµLar disµLphide link. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).