ELISA Alpha-fetoprotein Lens culinaris agglutiin 3 (AFP-L3)

Quantity:96T. Other Quantitys are also available. Please Inquire. Reactivity: (Homo sapiens) UniProt:N/A Abbreviation:AFP-L3 Alternative Names:N/A Application:ELISA Range:0.625-40 ng/mL Sensitivity:0.244 ng/mL Intra-AssayCV:≤7.3% Inter-AssayCV:≤9.2% Recovery:0,98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate AFP-L3 in samples. An antibody specific for AFP-L3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAFP-L3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for AFP-L3 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFP-L3 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:AFP-L3 is one of AFP fractions which is syntheQuantityd by malignant cell and comprise special oligosaccharide.It is a new generation of tumor marker for HCC and yields usefµL information on HCC for clinical decision making. Reports at home or abroad have showed that if the ratio of AFP-L3 increased over 10%, the risk of catching HCC had increased highly, and non-malignant diseases hardly syntheQuantity this AFP fraction(AFP-L3).AFP-L3 can be detected 9-12 months earlier and shows the remarkable precaution effect to HCC. AFP-L3 is the fucosylated variant of AFP produced specifically by HCC. AFP-L3 bears strong affinity to the sµgar chain of Lens cµLinaris, a type of lentil. AFP-L3 is less sensitive but more specific than AFP, and therefore cannot replace AFP. The value of AFP-L3 lies mainly in distinguishing HCC from other conditions when AFP is elevated. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).