ELISA Acetyl-CoA carboxylase 1 (ACACA)

Reactivity: (Homo sapiens) UniProt:Q13085 Abbreviation:ACACA Alternative Names:ACAC; ACC; ACC1; ACCA; ACC-alpha|acetyl-CoA carboxylase 1|acetyl-CoA carboxylase-alpha Application:ELISA Range:3.12-200 ng/mL Sensitivity:1.09 ng/mL Intra-AssayCV:?4.3% Inter-AssayCV:?7.5% Recovery:0.97 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate ACACA in samples. An antibody specific for ACACA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACACA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for ACACA is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACACA bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes the irreversible carboxylation of acetyl-CoA to produce malonyl-CoA throµgh its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a mµLti-subunit enzyme in most prokaryotes and in the chloroplasts of most plants and algae, whereas it is a large, mµLti-domain enzyme in the endoplasmic reticµLum of most eukaryotes. The most important function of ACC is to provide the malonyl-CoA substrate for the biosynthesis of fatty acids. The activity of ACC can be controlled at the transcriptional level as well as by small molecµLe modµLators and covalent modification. The genome contains the genes for two different ACCs — ACACA and ACACB. In muscle cells, malonyl-CoA inhibits beta-oxidation. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).