ELISA Alpha-2-antiplasmin (SERPINF2)

Reactivity: (Homo sapiens) UniProt:N/A Abbreviation:SERPINF2 Alternative Names:A2AP; AAP; ALPHA-2-PI; API; PLI; OTTHUMP00000115656|alpha-2-antiplasmin|alpha-2-plasmin inhibitor|serine (or cysteine) proteinase inhibitor; clade F (alpha-2 antiplasmin; pigment epithelium derived Application:ELISA Range:1.56-100 ng/mL Sensitivity:0.69 ng/mL Intra-AssayCV:?6.3% Inter-AssayCV:?8.5% Recovery:0.97 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SERPINF2 in samples. An antibody specific for SERPINF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SERPINF2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINF2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:An inherited hemorrhagic diathesis due to plasmin inhibitor deficiency was described by Koie et al. (1978) in a 25-year-old Okinawa man. He had suffered prolonged bleeding and ecchymoses after minor trauma, spontaneous joint hemorrhage, and one episode of hemothorax. The bleeding episodes were reduced in frequency and severity by an antiplasminic drµg. Laboratory abnormalities were limited to shortened eµglobµLin-lysis time and whole blood clot lysis time. No circµLating alpha-2-plasmin inhibitor was found in the plasma. Even thoµgh only one case was observed, autosomal recessive inheritance was undoubted because the parents were consanguineous and both had plasma antiplasmin levels about half normal. The authors called the condition Miyasato disease after the proband's surname. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).